Methods 3-one-12-hydroxy-13,14-ene-andrographolide was synthesized in a three-step route, including protecting primary alcohol of andrographolide by trityl ether, oxidating secondary alcohol and removing protecting group, etc. 方法通过三苯甲醚保护伯醇、氧化仲醇、脱去保护基的方法,合成3酮基12羟基13,14烯穿心莲内酯。